The Agilent HPLC-Chip/6210 TOF LC/MS Enables Highly Accurate Profiling of Peptide Maps for Differential Expression Studies
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چکیده
Liquid chromatography/mass spectrometry (LC/MS) based workflows, with their analytical power and potential throughput, play an important role in the discovery and validation of protein-based biomarkers. Current LC/MS workflows for biomarker discovery range from the classical shotgun proteomics approach to protein profiling strategies. However, no single LC/MS workflow has been adopted by the scientific community as the gold standard for biomarker research. Shotgun proteomic approaches utilize data-dependent MS/MS acquisition, which identifies only a subset of the actual proteins present in the entire sample; these proteins usually represent the higher abundance proteins that are not necessarily of biological significance in disease. As a result, extensive fractionation of complex samples may be necessary to identify meaningful protein biomarkers associated with disease, thus dramatically increasing the number of analyses required per sample. The Agilent HPLC-Chip/6210 TOF LC/MS Enables Highly Accurate Profiling of Peptide Maps for Differential Expression Studies Customer Success Story
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